Cubanana cristinae gen. et sp. nov. is described from eastern Cuba and its phylogenetic relationships are discussed. Males are characterized by presenting a denticulate subapical keel on the palpal bulbs, a nodule in the retrolateral side of the palpal tibiae and two-branched tibial spurs on legs I. Both sexes lack stridulating apparatus, possess urticating hairs of types I and III and the retrolateral side of the femora IV covered by a pad of ciliate hairs, in addition to the Theraphosinae synapomorphies. A list of currently recognized 49 genera of Theraphosinae is given, as well as data on its composition and depository institutions of type specimens of type species. Mygale nigrum Walckenaer 1837 is recovered as the type species of Homoeomma Ausserer 1871.