A new genus, Sinophaena Lin & Li, gen. nov., of the spider family Anyphaenidae Bertkau, 1878 is reported, with S. xiweni Lin & Li, sp. nov. (♀) from Sichuan, China as the type species. Sinophaena bivalva (Zhang & Song, 2004) comb. nov. is transferred from Anyphaena Sundevall, 1833. Further new species reported in the current paper include: Anyphaena grovyle Lin & Li, sp. nov. (♂♀, Hainan), A. sceptile Lin & Li, sp. nov. (♂♀, Hainan), A. shenzhen Lin & Li, sp. nov. (♂♀, Guangdong), A. tibet Lin & Li, sp. nov. (♂♀, Tibet), A. treecko Lin & Li, sp. nov. (♂♀, Hainan), Cybaeus fushun Lin & Li, sp. nov. (♀, Liaoning), C. huadian Lin & Li, sp. nov. (♀, Jilin), Eresus lishizheni Lin, Marusik & Li, sp. nov. (♂, Xinjiang), Alistra pikachu Lin & Li, sp. nov. (♂♀, Guangdong), Sinopesa gollum Lin & Li, sp. nov. (♂, Guangdong), Pimoa gagna Zhang & Li, sp. nov. (♀, India), P. nainital Zhang & Li, sp. nov. (♂♀, India), P. shoja Zhang & Li, sp. nov. (♂♀, India), Selenops crewsae Lin & Li, sp. nov. (♀, Sichuan), Sinopoda helii Wang & Li, sp. nov. (♂♀, Sichuan), Phlogiellus jiaxiangi Lin & Li, sp. nov. (♂♀, Guangxi), Episinus bonjovi Lin & Li, sp. nov. (♂♀, Yunnan), E. jiangweni Lin & Li, sp. nov. (♂♀, Yunnan), E. tongyani Lin & Li, sp. nov. (♂♀, Yunnan), Meotipa luoqiae Lin & Li, sp. nov. (♂, Yunnan), M. menglun Lin & Li, sp. nov. (♂, Yunnan), and M. zhengguoi Lin & Li, sp. nov. (♂, Yunnan). The female of Belisana yuexiu Yao & Li, 2020 is reported for the first time. All types are deposited in the Institute of Zoology, Chinese Academy of Sciences (IZCAS) in Beijing, China.