Acanthoscurria sacsayhuaman sp. nov. (Araneae: Theraphosidae) is described based on a male from Cusco,
Peru. The new species is characterized by the absence of stridulatory bristles on retrolateral face of palpal
trochanter. Moreover, it can be distinguished by morphology of the male palpal bulb and tibial apophysis of the
first pair of legs. The genus is recorded for the first time for Peru.