Two new species of Plesiopelma Pocock, 1901 from northern Argentina are described and diagnosed based on males and habitat descriptions are presented. Males of Plesiopelma paganoi sp. nov. differ from most of species by the absence of spiniform setae on the retrolateral face of cymbium, aspect of the palpal bulb. Plesiopelma aspidosperma sp. nov. differs from most species of the genus by the presence of spiniform setae on the retrolateral face of cymbium and it can be distinguished from P. myodes Pocock, 1901, P. longisternale (Schiapelli & Gerschman, 1942) and P. rectimanum (Mello-Leitão, 1923) by the separated palpal bulb keels and basal nodule of metatarsus I very developed. It differs from P. minense (Mello-Leitão, 1943) by the shape of the palpal bulb and basal nodule on metatarsus I well developed. Specimens were captured in Salta province, Argentina, inhabiting high cloud forests of Yungas eco-region.
A new species of Eupalaestrus Pocock, 1901 from northern Argentina is described and illustrated. Males and females of Eupalaestrus larae
sp. nov. differ from those all other species of the genus by the color with distinct two parallel longitudinal stripes on the femora, patellae, tibiae and one longitudinal stripe reaching half of metatarsi; the presence of a thickened femur and tibia IV; a straight embolus of the male palpal bulb and retrolateral keel pronounced. Specimens were captured in Chaco province, inhabiting unflooded flat grasslands open areas inside forest in transitional Chaco eco-region.